Anti-5-methylcytosine (5-mC) antibody [5MC-CD]
| Name | Anti-5-methylcytosine (5-mC) antibody [5MC-CD] |
|---|---|
| Supplier | Abcam |
| Catalog | ab73938 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgM |
| Clone | 5MC-CD |
| Applications | Southern blot ICC/IF ICC/IF FC |
| Antigen | Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine Serum Albumin (BSA) |
| Description | Mouse Monoclonal |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab73938 staining 5-Methyl Cytosine in human sperm cells by Flow Cytometry. Cells were fixed with &0% ethanol and permeabilized with 5% tween. The sample was incubated with the primary antibody (1/100 in PBST) for 30 minutes at 27°C. A Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/300) was used as the secondary antibody. See Abreview
Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with ab73938 and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells
ab73938 staining 5-Methyl Cytosine in murine PN5 zygotes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with methanol/acetic acid (3:1) and blocked with 30% serum for overnight a 4°C. Samples were incubated with primary antibody (1/100 in diluent) for 18 hours at 4°C before detection with a fluorescent secondary antibody.
Product References
Epigenetic chromatin modifications in barley after mutagenic treatment. - Epigenetic chromatin modifications in barley after mutagenic treatment.
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Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum - Tissue-specific epigenetic modifications in root apical meristem cells of Hordeum
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Persistence of cytosine methylation of DNA following fertilisation in the mouse. - Persistence of cytosine methylation of DNA following fertilisation in the mouse.
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Mechanism of glycyrrhizic acid inhibition of Kaposi's sarcoma-associated - Mechanism of glycyrrhizic acid inhibition of Kaposi's sarcoma-associated
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Concomitant heterochromatinisation and down-regulation of gene expression unveils - Concomitant heterochromatinisation and down-regulation of gene expression unveils
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The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to - The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to
Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H. Nature. 2007 Dec 6;450(7171):908-12. Epub 2007 Nov 11.
A chloroplast-resident DNA methyltransferase is responsible for hypermethylation - A chloroplast-resident DNA methyltransferase is responsible for hypermethylation
Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H. Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5925-30.
Detection of heavy methylation in human repetitive DNA subsets by a monoclonal - Detection of heavy methylation in human repetitive DNA subsets by a monoclonal
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