Human neuroblastoma SH-SY5Y cells stained with chicken anti-NF-H (red) and mouse monoclonal to fibrillarin 38F3 (green). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000. Cultures were processed using our standard fixation and staining procedure (in protocol section).

Rat Neurons stained with NFH (red) and rabbit polyclonal to GFAP(green). The NF-H antibody was used at a dilution of 1/100000 and the GFAP at a dilution of 1:2,000 using our standard fixation and staining procedure (see protocol section).

ab4680 at 1/10000 dilution staining neurofilament in rat skin tissue by immunohistochemistry (frozen sections). Sections were fixed using Bouin's Fixative and permeabilized in 0.1% Triton X-100 prior to blocking in 10% serum for 1 hour at 24°C and then incubated with ab4680 for 24 hours at 4°C. A donkey polyclonal to chicken IgY, diluted 1/100 was used as the secondary antibody.See Abreview

ab4680 staining 200 kD Neurofilament Heavy (green) in murine embryoid bodies by Immunocytochemistry/ Immunofluorescence. Nuclei were visualized with DAPI.