All lanes : Anti-2 Hydroxy phytanoyl CoA lyase antibody [EPR16812] (ab197025) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Rat brain lysateLane 4 : Rat heart lysateLane 5 : C6 (Rat glial tumor cells) whole cell lysatesLane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysatesLane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysatesLane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysatesLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

2 Hydroxy phytanoyl CoA lyase was immunoprecipitated from HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab197025 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab197025 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract (Input) 10 µg. Lane 2: ab197025 IP in HeLa whole cell extract. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab197025 in HeLa whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.