Anti-2 Cys Peroxiredoxin antibody [6E5]
| Name | Anti-2 Cys Peroxiredoxin antibody [6E5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16765 |
| Prices | $380.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6E5 |
| Applications | ELISA WB FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Escherichia coli |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | PRDX4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab16765 stained HCT116 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16765, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM
![Overlay histogram showing HeLa cells stained with ab16765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16765, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_1/362_2-Cys-Peroxiredoxin-Primary-antibodies-ab16765-1.jpg)
Overlay histogram showing HeLa cells stained with ab16765 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16765, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
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Induction of sulfiredoxin expression and reduction of peroxiredoxin - Induction of sulfiredoxin expression and reduction of peroxiredoxin
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Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses. - Synaptic NMDA receptor activity boosts intrinsic antioxidant defenses.
Papadia S, Soriano FX, Leveille F, Martel MA, Dakin KA, Hansen HH, Kaindl A, Sifringer M, Fowler J, Stefovska V, McKenzie G, Craigon M, Corriveau R, Ghazal P, Horsburgh K, Yankner BA, Wyllie DJ, Ikonomidou C, Hardingham GE. Nat Neurosci. 2008 Apr;11(4):476-87.
The 2-cys peroxiredoxin-deficient Listeria monocytogenes displays impaired growth - The 2-cys peroxiredoxin-deficient Listeria monocytogenes displays impaired growth
Kim KP, Hahm BK, Bhunia AK. Curr Microbiol. 2007 May;54(5):382-7. Epub 2007 May 5.