Anti-160 kD Neurofilament Medium antibody [NF-09]
| Name | Anti-160 kD Neurofilament Medium antibody [NF-09] |
|---|---|
| Supplier | Abcam |
| Catalog | ab7794 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | NF-09 |
| Applications | ELISA IHC-F ICC/IF IHC-P WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Bovine, Cat, Human, Pig, Broad species, Mammalian |
| Antigen | Pellet of pig brain cold stable proteins after depolymerization of microtubules |
| Description | Mouse Monoclonal |
| Gene | NEFM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab7794 at a dilution of 1/1000, staining 160 kD Neurofilament Medium (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Image taken with a 40x objective. No labeling observed following omission of primary antibody.Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.
Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794. Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.
Product References
Characterization of mesenchymal progenitor cell populations from non-epithelial - Characterization of mesenchymal progenitor cell populations from non-epithelial
Matsumura S, Higa K, Igarashi T, Takaichi S, Tonogi M, Shinozaki N, Shimazaki J, Yamane GY. Oral Dis. 2015 Apr;21(3):361-72.
Subjects harboring presenilin familial Alzheimer's disease mutations exhibit - Subjects harboring presenilin familial Alzheimer's disease mutations exhibit
Roher AE, Maarouf CL, Malek-Ahmadi M, Wilson J, Kokjohn TA, Daugs ID, Whiteside CM, Kalback WM, Macias MP, Jacobson SA, Sabbagh MN, Ghetti B, Beach TG. Am J Neurodegener Dis. 2013 Sep 18;2(3):187-207. eCollection 2013.
Investigation of cytoskeleton proteins in neurons of the cat lateral geniculate - Investigation of cytoskeleton proteins in neurons of the cat lateral geniculate
Duffy KR, Crowder NA, LeDue EE. J Comp Neurol. 2012 Jan 1;520(1):186-99.
Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics. - Dental pulp stem cells differentiation reveals new insights in Oct4A dynamics.
Ferro F, Spelat R, D'Aurizio F, Puppato E, Pandolfi M, Beltrami AP, Cesselli D, Falini G, Beltrami CA, Curcio F. PLoS One. 2012;7(7):e41774.
Ciliary neurotrophic factor recruitment of glucagon-like peptide-1 mediates - Ciliary neurotrophic factor recruitment of glucagon-like peptide-1 mediates
Belsham DD, Fick LJ, Dalvi PS, Centeno ML, Chalmers JA, Lee PK, Wang Y, Drucker DJ, Koletar MM. FASEB J. 2009 Dec;23(12):4256-65.
Isolation of multipotent neural crest-derived stem cells from the adult mouse - Isolation of multipotent neural crest-derived stem cells from the adult mouse
Yoshida S, Shimmura S, Nagoshi N, Fukuda K, Matsuzaki Y, Okano H, Tsubota K. Stem Cells. 2006 Dec;24(12):2714-22. Epub 2006 Aug 3.
Monoclonal antibody NF-09 specific for neurofilament protein NF-M. - Monoclonal antibody NF-09 specific for neurofilament protein NF-M.
Draberova E, Sulimenko V, Kukharskyy V, Draber P. Folia Biol (Praha). 1999;45(4):163-5.