ImmunohistochemistryAnti-TRPV4:
Cat. No. T9075: Immunohistochemical staining of Rat dorsal root ganglion (DRG) frozen sections using Anti-TRPV4 antibody.
A. TRPV4 (red) in DRG neurons.
B. Staining with Mouse Anti-Parvalbumin (green) in the same DRG section.
C. Confocal merge of TRPV4 and Parvalbumin demonstrates colocalization.
ImmunocytochemistryAnti-TRPV4:
Cat. No. T9075: Immunocytochemical staining of paraformaldehyde-fixed and permeabilized Rat dorsal root ganglion (DRG) primary culture.
A, D. Staining using Anti-TRPV4 antibody, (1:500), followed by Goat Anti-Rabbit-AlexaFluor-555 secondary antibody.
B, E. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.
C. Merged image of panels A and B.
F. Merged image of panels D and E.
ImmunoblottingAnti-TRPV4:
Cat. No. T9075: Western blot analysis of ND7/23 cell line lysate.
Lanes1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.
ImmunoblottingAnti-TRPV4:
Cat. No. T9075: Western blot analysis of Rat brain lysates.
Lanes1. Anti-TRPV4 antibody, (1:200).
2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.