ImmunoblottingLysate of HEK-293T cells overexpressing human BAAT was separated on SDS-PAGE, blotted with Anti-BAAT (326-340) (
Cat. No. B8812) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1.Antibody dilution 1:500
2. Antibody dilution 1:500 + BAAT immunizing peptide (human, 326-340)
ImmunofluorescenceAnti-BAAT (326-340):
Cat. No. B8812: Immunofluorescence of HUVEC cells using BAAT (326-340), Cat. No. B8812 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to
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ChartAnti-BAAT (326-340):
Cat. No. B8812: Intensity analysis of Anti-BAAT (326-340) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.