Western blot detection of Adducin phosphorylation in NIH/3T3 cells. Blots were probed with Adducin (H-16): sc-11884 (A) and p-Adducin (Ser 662)-R: sc-12614-R (B,C). The antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
p-Adducin (Ser 662)-R: sc-12614-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin β phosphorylation in SK-N-MC whole cell lysate.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin β prosphorylation in non-transfected 293T: sc-117752 (A) and human Adducin β transfected 293T: sc-117077 (B) whole cell lysates and mouse brain tissue extract (C).
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin γ phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin γ transfected: sc-118249 (B) 293T whole cell lysates.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin phosphorylation in NIH/3T3 whole cell lysate.
p-Adducin (Ser 662): sc-12614. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin α transfected: sc-118250 (B) 293T whole cell lysates.
Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662): sc-12614 (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F).
Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F).
Western blot analysis of Adducin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) HL-60 whole cell lysates. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A,B) and Adducin α (H-100): sc-25731 (C,D).
Western blot analysis of Adducin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A,B) and Adducin α (H-100): sc-25731 (C,D).