Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant p38α treated (B,E) and human recombinant p38α and lambda protein phosphatase (sc-200312A) treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Thr 74)-R: sc-16322-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant ERK 2 treated (B,E) and human recombinant ERK 2 and lambda protein phosphatase (sc-200312A) treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Thr 74)-R: sc-16322-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).