Bcl-2 (C-2) PE: sc-7382 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2866.
Western blot analysis of Bcl-2 expression in HL-60 (A,D), Jurkat (B,E) and WEHI-231 (C,F) whole cell lysates. Antibodies tested include Bcl-2 (C-2): sc-7382 (A-C) and Bcl-2 (100): sc-509 (D-F).
Bcl-2 (C-2): sc-7382. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon tissue showing cytoplasmic staining of lymphoid cells (A). Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic localization (B).
Bcl-2 (C-2) Alexa Fluor 647: sc-7382 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-24636.
Bcl-2 (C-2): sc-7382. Western blot analysis of Bcl-2 expression in non-transfected 293T: sc-117752 (A), mouse Bcl-2 transfected 293T: sc-118779 (B) and Jurkat (C) whole cell lysates.
Western blot analysis of Bcl-2 phosphorylation in untreated (A,C), and paclitaxel treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-Bcl-2 (74.Ser 70): sc-135757 (A,B) and Bcl-2 (C-2): sc-7382 (C,D).
Bcl-2 (C-2): sc-7382. Western blot analysis of Bcl-2 expression in non-transfected: sc-117752 (A) and human Bcl-2 transfected: sc-176463 (B) 293T whole cell lysates.
Bcl-2 (C-2) FITC: sc-7382 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG
1: sc-2855.
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant p38α treated (B,E) and human recombinant p38α and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Thr 74)-R: sc-16322-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant p38α treated (B,E) and human recombinant p38α and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-p-Bcl-2 (Ser 87)-R: sc-16323-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant p38α treated (B,E) and human recombinant p38α and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Thr 56)-R: sc-16321-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant ERK 2 treated (B,E) and human recombinant ERK 2 and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Thr 74)-R: sc-16322-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant ERK 2 treated (B,E) and human recombinant ERK 2 and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (Ser 87)-R: sc-16323-R (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), human recombinant ERK 2 treated (B,E) and human recombinant ERK 2 and lambda protein phosphatase treated (C,F) human recombinant Bcl-2 fusion proteins. Antibodies tested include p-Bcl-2 (A-3): sc-377513 (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), paclitaxel treated (B,E) and paclitaxel and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-Bcl-2 (367.Ser 70): sc-293128 (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), paclitaxel treated (B,E) and paclitaxel and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-Bcl-2 (662.Ser 70): sc-293129 (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).
Bcl-2 (C-2): sc-7382. Western blot analysis of Bcl-2 expression in Jurkat whole cell lysate.
Western blot analysis of Bcl-2 phosphorylation in untreated (A,D), ERK2 treated (B,E) and ERK2 and lambda protein phosphatase (sc-200312A) treated (C,F) human recombinant Bcl-2. Antibodies tested include p-Bcl-2 (A-11): sc-377554. (A,B,C) and Bcl-2 (C-2): sc-7382 (D,E,F).