Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-SAPAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Rat brain lysates probed with SAPAP4/DLGAP4 Polyclonal Antibody, unconjugated (bs-12140R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SAPAP4/DLGAP4 Polyclonal Antibody, Unconjugated (bs-12140R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes and DAPI for nuclei staining.