Artemin was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse Artemin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1085) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.