![Flow Cytometry: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Analysis using the FITC conjugate of NBP1-43247. Mouse thiogylcolate-induced peritoneal exudate cells (PECs) were stimulated with LPS in the presence of Brefeldin A for 22 hours. The cells were surface stained with Anti-Mouse CD11b PE and then intracellularly stained with Rat IgG2b kappa Isotype Control FITC (left) or Anti-Mouse IL-1 alpha FITC (right).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/17471_IL-1-alpha-IL-1F1-Antibody-(ALF-161)-Flow-Cytometry-NBP1-43247-img0004.jpg)
Flow Cytometry: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Analysis using the FITC conjugate of NBP1-43247. Mouse thiogylcolate-induced peritoneal exudate cells (PECs) were stimulated with LPS in the presence of Brefeldin A for 22 hours. The cells were surface stained with Anti-Mouse CD11b PE and then intracellularly stained with Rat IgG2b kappa Isotype Control FITC (left) or Anti-Mouse IL-1 alpha FITC (right).
![ELISA: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_10/17472_IL-1-alpha-IL-1F1-Antibody-(ALF-161)-ELISA-NBP1-43247-img0002.jpg)
ELISA: IL-1 alpha/IL-1F1 Antibody (ALF-161) [NBP1-43247] - Mouse splenocytes (500,000 cells per well) were activated with LPS (1 ug/ml) for 48 hrs in mouse IL-1 alpha ELISPOT assay. Control well is medium alone.