![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Analysis using the Biotin conjugate of NBP1-77775. Mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used in Detection ofJurkat and Raji cells or 3% BSA.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/12115_Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-77775-img0005.jpg)
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Analysis using the Biotin conjugate of NBP1-77775. Mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used in Detection ofJurkat and Raji cells or 3% BSA.
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/12116_Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-77775-img0003.jpg)
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA.
![Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_9/12117_Adenosine-Deaminase-ADA-Antibody-Western-Blot-NBP1-77775-img0004.jpg)
Western Blot: Adenosine Deaminase/ADA Antibody [NBP1-77775] - Lane 1: Adenosine Deaminase (Calf Spleen). Lane 2: None. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.