
Western blot analysis of extracts from various cell lines using Phospho-Vimentin (Ser39) Antibody (upper), Vimentin (D21H3) XP ® Rabbit mAb #5741 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Publications have shown that the human breast carcinoma cell line, ZR-75-1, does not express vimentin.

Western blot analysis of Hs578T cell extracts, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Vimentin (Ser39) Antibody (upper) and Vimentin (D21H3) XP ® Rabbit mAb #5741 (lower).

Western blot analysis of SK-LMS-1 cell extracts, serum-starved for 16 hr and treated with LY294002 #9901 (50 μM, 30 min; +) and/or Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 30 min; +), using Phospho-Vimentin (Ser39) Antibody (upper), Vimentin (D21H3) XP ® Rabbit mAb #5741 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).