Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb

Name	Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	12569
Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D5A2D
Applications	WB ICC/IF FC
Species Reactivities	Human, Mouse, Rat
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser83 of human vimentin protein.
Description	Rabbit Monoclonal
Gene	VIM
Supplier Page	Shop

Product images

Western blot analysis of extracts from HeLa cells, untreated (-), or treated with either hydroxyurea (4 mM, G1/S arrested; +), or Paclitaxel #9807 (100 nM, G2/M arrested; +) for 20 hr, using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb (upper) and Vimentin (D21H3) XP ® Rabbit mAb #5741 (lower).

Confocal immunofluorescent analysis of HeLa cells using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 (DNA content). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.