Vitamin D3 Receptor (D2K6W) Rabbit mAb

Name	Vitamin D3 Receptor (D2K6W) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	12550
Prices	$250.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	D2K6W
Applications	WB IP IHC-P ChIP
Species Reactivities	Human, Mouse, Hamster, Bovine, Pig, Horse
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human vitamin D3 receptor isoform A protein.
Description	Rabbit Monoclonal
Gene	VDR
Supplier Page	Shop

Product images

Western blot analysis of extracts from various cell lines using Vitamin D3 Receptor (D2K6W) Rabbit mAb.

Western blot analysis of kidney tissue extracts from Vdr (+/+) and Vdr (-/-) mice using Vitamin D3 Receptor (D2K6W) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (Vdr (+/+) and Vdr (-/-) kidneys were kindly provided by Dr. Marie Demay, Massachusetts General Hospital).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human vitamin D receptor isoform A (hVDRA-Myc/DDK; +), full-length human pregnane X receptor (hPXR-Myc/DDK; +), and full-length human constitutive androstane receptor (hCAR-Myc/DDK; +), using Vitamin D3 Receptor (D2K6W) Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).

Immunoprecipitation of vitamin D3 receptor from T-47D cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Vitamin D3 Receptor (D2K6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Vitamin D3 Receptor (D2K6W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vitamin D3 Receptor (D2K6W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Vitamin D3 Receptor (D2K6W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse kidney, Vdr (+/+; left) or Vdr (-/-; right), using Vitamin D3 Receptor (D2K6W) Rabbit mAb (Tissues courtesy of Dr. Marie Demay, Massachusetts General Hospital).

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 LS180 cells treated with calcitriol (10nM, 3 hours) and either 10 μl of Vitamin D3 Receptor (D2K6W) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human c-Fos Upstream Primers #25661, human UCA1 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.