![Western Blot: RC74 Antibody [NB100-589] - Nuclear extract (25 mcg for WB in A; 5 mg for IP in B) from HeLa cells. Affinity purified rabbit anti-RC74 antibody used at 0.05 mcg/ml for WB in A and at 4 mcg/5 mg extract for IP in B. RC74 was also immunoprecipitated using rabbit anti-RC74 antibody NB100-590 at 4 mcg/5 mg extract. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the bands that are RC74 was verified by mass spectrometry.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/24081_RC74-Antibody-Western-Blot-NB100-589-img0001.jpg)
Western Blot: RC74 Antibody [NB100-589] - Nuclear extract (25 mcg for WB in A; 5 mg for IP in B) from HeLa cells. Affinity purified rabbit anti-RC74 antibody used at 0.05 mcg/ml for WB in A and at 4 mcg/5 mg extract for IP in B. RC74 was also immunoprecipitated using rabbit anti-RC74 antibody NB100-590 at 4 mcg/5 mg extract. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the bands that are RC74 was verified by mass spectrometry.
![Immunohistochemistry: RC74 Antibody [NB100-589] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RC74 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_8/24082_RC74%2520Antibody-Immunohistochemistry-NB100-589-img0005.jpg)
Immunohistochemistry: RC74 Antibody [NB100-589] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RC74 used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB