Confocal immunofluorescent analysis of C2C12 cells, insulin-treated (left) or treated with LY294002 #9901, Rapamycin #9904, and U0126 #9903 (right), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate).