Western Blot: RBM22 Antibody [NBP2-22276] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22276 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: RBM22 Antibody [NBP2-22276] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-RBM22 antibody NBP2-22276 used for IP at 6 ug/mg lysate. RBM22 was also immunoprecipitated by rabbit anti-RBM22 antibody NBP2-22275. For blotting immunoprecipitated RBM22, NBP2-22276 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.