Western Blot: beta II Tubulin B Antibody (AT5B3) [NBP1-74029] - PAGE, transferred to PVDF membrane and probed with anti-human TUBB2b (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/Immunofluorescence: beta II Tubulin B Antibody (AT5B3) [NBP1-74029] - Immunofluorescence of human SW480 cells stained with monoclonal anti-human TUBB2b antibody (1:250) with Texas Red (Red). Arrow head: Mitotic cell, Arrow: MTOC (microtubule organizing center).