Western Blot: EYA4 Antibody [NBP1-41100] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EYA4 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. EYA4 was also immunoprecipitated by rabbit anti-EYA4 antibody NBP1-41099, which recognizes an upstream epitope.
Immunohistochemistry: EYA4 Antibody [NBP1-41100] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-EYA4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: EYA4 Antibody [NBP1-41100] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250 Detection: DAB staining using Immunohistochemistry Accessory Kit.