HEK293 human embryonic kidney cell line transfected with human Claudin-8 was stained with Mouse Anti-Human Claudin‑8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Caki‑2 human clear cell carcinoma epithelial cell line was stained with Mouse Anti-Human Claudin‑8 Monoclonal Antibody (Catalog # MAB5275, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.