Western blot shows lysates of NR8383 rat alveolar macrophage cell line, K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, M1 mouse myeloid leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Nucleoporin NUP85 Monoclonal Antibody (Catalog # MAB5976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Nucleoporin NUP85 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.