Western Blot: BCKDHB Antibody [NBP2-29984] - Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunocytochemistry/Immunofluorescence: BCKDHB Antibody [NBP2-29984] - Confocal immunofluorescent analysis of (N-term)(NBP2-29984) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry: BCKDHB Antibody [NBP2-29984] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western Blot: BCKDHB Antibody [NBP2-29984] - Western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).
Western Blot: BCKDHB Antibody [NBP2-29984] - Western blot analysis of BCKDHB (arrow) using rabbit polyclonal (N-term) (NBP2-29984). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.