Western Blot: Orc2 Antibody [NBP1-46175] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-ORC2 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ORC2 was also immunoprecipitated by rabbit anti-ORC2 antibody NBP1-46175, which recognizes an upstream epitope.
Immunohistochemistry: Orc2 Antibody [NBP1-46175] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ORC2 used at a dilution of 1:1,000 (0.2ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit.
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM7 (NB100-252) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.
Proximity Ligation Assay: Orc2 Antibody [NBP1-46175] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPA2 (NBP2-22232) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.