Recombinant Equine IFN‑ gamma (Catalog # 1586‑HG) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose‑dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Equine IFN‑ gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586). The ND50 is typically 0.25-1.25 µg/mL.
IFN‑ gamma was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) treated with Calcium Ionomycin and PMA using Goat Anti-Equine IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1586) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.