Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 59 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.