![Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/5681_APRIL-TNFSF13-Antibody-(Aprily-8)-Western-Blot-NBP1-97587-img0006.jpg)
Western Blot: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL.Method: 10ug of protein was applied to the gel. Revealed with Aprily-8 (1ug/ml) and HRP-coupled anti-mouse secondary antibody (1:4000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).
![Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/5682_APRIL-TNFSF13-Antibody-(Aprily-8)-Immunohistochemistry-Paraffin-NBP1-97587-img0005.jpg)
Immunohistochemistry-Paraffin: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.
![Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/5683_APRIL-TNFSF13-Antibody-(Aprily-8)-Flow-Cytometry-NBP1-97587-img0004.jpg)
Flow Cytometry: APRIL/TNFSF13 Antibody (Aprily-8) [NBP1-97587] - Analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x10^5) were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10ug/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.