![Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/1411_SP1-Antibody-Immunohistochemistry-Paraffin-NB600-232-img0008.jpg)
Immunohistochemistry-Paraffin: SP1 Antibody [NB600-232] - Sample: FFPE sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Sp1 used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
![Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 on HeLa whole cell lysate using NB600-232. Subsequently, NB600-232 was used at 0.04 mcg/ml for WB.](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/1412_SP1-Antibody-Western-Blot-NB600-232-img0001.jpg)
Western Blot: SP1 Antibody [NB600-232] - Detection of Human SP1 on HeLa whole cell lysate using NB600-232. Subsequently, NB600-232 was used at 0.04 mcg/ml for WB.
![Western Blot: SP1 Antibody [NB600-232] - Samples : A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. Antibody : A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/ml for WB. Detection : Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).](http://www.bioprodhub.com/system/product_images/ab_products/5/sub_7/1413_SP1-Antibody-Western-Blot-NB600-232-img0006.jpg)
Western Blot: SP1 Antibody [NB600-232] - Samples : A. Whole cell lysate (50 ug for E; 10 ug for T) from HEK 293T cells that were mock transfected (E) or transfected with an Sp1 expression construct (T). B. Whole cell lysate (~700 ug) from HEK 293T cells. Antibody : A. Affinity purified rabbit anti-Sp1 antibody used at the indicated concentrations. B. SP1 was immunoprecipitated using affinity purified rabbit anti-SP1 antibodies BL937, using each antibody at 3.75 ug/mg lysate. Subsequently, was used at 0.04 ug/ml for WB. Detection : Chemiluminescence with an exposure time of 10 seconds (A) or 1 min (B).