Immunocytochemistry/Immunofluorescence: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody , 1ug/ml) overnight at +4C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM

Immunohistochemistry: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Analysis of paraffin-embedded human placenta tissue.

Flow Cytometry: Musashi-2 Antibody (EP1305Y) [NBP1-42029] - Overlay histogram showing HeLa cells stained with NBP1-42029 (red line).