Western blot shows 25 ng of Recombinant Mouse IL‑10 (Catalog # 417-ML), Recombinant Human IL‑10 (Catalog # 217-IL), Recombinant Rat IL‑10 (Catalog # 522-RLB), and Recombinant Human IL‑26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Recombinant Rat IL‑10 (Catalog # 522-RL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50 is typically 0.3-1.0 µg/mL.