Western blot analysis of extracts from various cell lines using Skp2 (D3G5) XP ® Rabbit mAb.
Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human Skp2 (hSkp2-Myc/DDK, +), using Skp2 (D3G5) XP ® Rabbit mAb.
Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or shSkp2, using Skp2 (D3G5) XP ® Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Skp2 (D3G5) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using Skp2 (D3G5) XP ® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using Skp2 (D3G5) XP ® Rabbit mAb.
Confocal immunofluorescent analyis of HeLa cells expressing either a non-targeting shRNA (shNT; left) or shSkp2 (right) using Skp2 (D3G5) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red) (Cell lines kindly provided by Dr. Wenyi Wei, Harvard Medical School.)