Phospho-Skp2 (Ser64) Antibody

Name	Phospho-Skp2 (Ser64) Antibody
Supplier	Cell Signaling Technology
Catalog	14865
Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal
Applications	WB IP
Species Reactivities	Human
Antigen	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser64 of human Skp2 protein
Description	Rabbit Polyclonal
Gene	SKP2
Supplier Page	Shop

Product images

Western blot analysis of extracts from HeLa cells expressing either non-targeting shRNA (shNT) or Skp2 shRNA (shSkp2) using Phospho-Skp2 (Ser64) Antibody (upper), Skp2 (D3G5) XP ® Rabbit mAb #2652 (middle), and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing DDK-tagged full-length, human Skp2 wild-type protein (hSkp2-WT-DDK; +) or DDK-tagged full-length, human Skp2 S64A protein (hSkp2-S64A-DDK; +), using Phospho-Skp2 (Ser64) Antibody (upper) and DYKDDDDK Tag Antibody #2368 (lower).

Western blot analysis of extracts from 293T and HeLa cells using Phospho-Skp2 (Ser64) Antibody. The phospho-specificity of the antibody was verified by pre-incubating the antibody with no peptide (left), Skp2 non-phosphopeptide (center), and Skp2 (Ser64) phosphopeptide (right).

Immunoprecipitation of phosphorylated Skp2 from 293T cell extracts using Normal Rabbit IgG #2729 (lane 2) or Phospho-Skp2 (Ser64) Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Phospho-Skp2 (Ser64) Antibody.