B7-H1/PD-L1/CD274 Antibody

Name	B7-H1/PD-L1/CD274 Antibody
Supplier	Novus Biologicals
Catalog	NBP1-76769
Prices	$129.00, $399.00
Sizes	25 µg, 100 µg
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	WB ELISA FC ICC/IF IHC IHC-P
Species Reactivities	Human, Rat
Antigen	Antibody was raised against a 17 amino acid synthetic peptide from near the center of human PD-L1. The immunogen is located within amino acids 60 - 110 of PD-L1.
Purity/Format	Immunogen affinity purified
Blocking Peptide	PD-L1/B7-H1 Blocking Peptide
Description	Rabbit Polyclonal
Gene	CD274
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Western Blot: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of PD-L1 in Raji cell lysate with PD-L1 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/Immunofluorescence: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - ICC/IF analysis of PD-L1 protein in A549 cells which were stably transfected with human-PD-L1-GFP construct. PD-L1 antibody NBP1-76769 was used at 1:200 dilution which followed incubation of cells with Cy3-conjugated secondary antibody (see red colored surface staining of PDL1). Green color in the image represents GFP-PDL1 while nuclear bound DAPI is in blue color. This image was submitted by Anudari Letian from Weill Cornell Medical College (NY City, USA) via product review.

Immunohistochemistry-Paraffin: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human tonsil tissue with PD-L1 antibody at 5 ug/ml.

Flow Cytometry: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Analysis of A-20 cells using B7-H1/PD-L1/CD274 antibody at 0.5 ug/ml. Green: Isotype control. Red : B7-H1/PD-L1/CD274 antibody.

Immunocytochemistry/Immunofluorescence: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Human Heart cells.

Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody [NBP1-76769] - Staining of human heart tissue with antibody at 2.5 ug/mL.