IRE1 alpha Antibody

Name	IRE1 alpha Antibody
Supplier	Novus Biologicals
Catalog	NB100-2324
Prices	$99.00, $299.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB ICC/IF IHC IHC-P
Species Reactivities	Human, Rat
Antigen	A synthetic peptide within the human IRE1 alpha protein (within residues 700-800). [Swiss-Prot #O75460]
Purity/Format	Immunogen affinity purified
Blocking Peptide	IRE1 alpha Peptide
Description	Rabbit Polyclonal
Gene	ERN1
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Western Blot: IRE1 alpha Antibody [NB100-2324] - Western Blot detection of Total IRE1 Alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA.

Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - IHC-P analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300 dilution. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. A few cells, potentially with active UPR, showed nuclear positivity also while the signal was negligible in cells at base of crypts and lamina propria.

Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody [NB100-2324] - The IRE-1 alpha antibody was tested in HeLa cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - IHC-P analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300 dilution. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. Some cells, most likely with active UPR, depicted nuclear positivity also while the signal was negligible in crypts cells and lamina propria.