Histone H3 Antibody

Name	Histone H3 Antibody
Supplier	Novus Biologicals
Catalog	NB500-171
Prices	$129.00, $329.00
Sizes	25 µl, 100 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB ChIP ICC/IF IHC IHC-P
Species Reactivities	Human, Rat, Yeast, C. elegans, Chimpanzee, Plant
Antigen	A synthetic peptide made to an N-terminal portion of the human Histone H3 protein (between residues 100-150) [UniProt P68431]
Purity/Format	Immunogen affinity purified
Description	Rabbit Polyclonal
Gene	HIST1H3F
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Image from verified customer review.

Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.

Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody (No Ab) was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.

Western Blot: Histone H3 Antibody [NB500-171] - Western blot analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100.

Western Blot: Histone H3 Antibody [NB500-171] - HeLa (lane 1) or S. cerevisiae (lane 2) whole cell lysates. Histones purified from HeLa cells (lane 3).

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded rat pancreas.

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded rat colon.

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded mouse brain.

Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in formalin fixed, paraffin-embedded mouse testis.