Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Sara siRNA I (+) #13542, or SignalSilence ® Sara siRNA II #13553 (+), using Sara (D5X4F) Rabbit mAb (upper) or α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower). The Sara (D5X4F) Rabbit mAb confirms silencing of Sara expression, while the α-Actinin (D6F6) XP ® Rabbit mAb is used as a loading control.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human Sara protein (hSara; +), using Sara (D5X4F) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Sara (D5X4F) Rabbit mAb.
Immunoprecipitation of Sara from SH-SY5Y cells using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Sara (D5X4F) Rabbit mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Sara (D5X4F) Rabbit mAb.