Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb

Name	Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	4857
Prices	$307.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	91B2
Applications	WB IHC-P IHC-F ICC/IF
Species Reactivities	Human, Mouse, Rat
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
Description	Rabbit Monoclonal
Gene	RPS6
Supplier Page	Shop

Product images

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100ng/ml, 20 min), using Phospho-S6 Ribosomal protein (Ser235/236) (91B2) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb in the presence of control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

Immunohistochemical analysis of frozen U-87MG xenograft, showing cytoplasmic localization using Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb.

Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).