Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb

Name	Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb
Supplier	Cell Signaling Technology
Catalog	4856
Prices	$309.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone	2F9
Applications	WB ICC/IF FC
Species Reactivities	Human, Mouse, Rat, Monkey
Antigen	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser235 and Ser236 of human ribosomal protein S6.
Description	Rabbit Monoclonal
Gene	RPS6
Supplier Page	Shop

Product images

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated (100 ng/ml, 20 min), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).

Confocal immunofluorescent images of HeLa cells serum-starved for 20 hrs (left), 20% serum-treated (center), or 20% serum-treated after preincubation with Rapamycin (FRAP/mTOR Inhibitor) #9904 and labeled with Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Flow cytometric analysis of Jurkat cells, untreated (green), or LY294002, Wortmannin and U0126-treated (blue), using Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb compared to a nonspecific negative control antibody (red).