Western blot analysis of extracts from 293 cells, untreated or treated with 20% FBS for the indiccated time, using , Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (upper) or Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 (lower).
Immunohistochemical staining of paraffin-embedded human melanoma, showing cytoplasmic localization of phosphorylated S6 ribosomal protein, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or calf-intestinal phosphatase (CIP) treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or rapamycin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Imunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody preincubated with control peptide (left) or Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide #1220 (right).
Immunohistochemical analysis of frozen U87MG xenograft, using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody.
Confocal immunofluorescent analysis of C2C12 cells, either U0126/LY294002/Rapamycin-treated (left) or insulin-treated (right), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of NIH/3T3 cells, U0126- and Rapamycin-treated (blue) or serum-treated (green), using Phospho-S6 Ribosomal Protein (Ser235/236) Antibody compared to a nonspecific negative control antibody (red).