Formalin-fixed and paraffin-embedded human lung carcinoma reacted with TRIP13 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of TRIP13 (arrow) using rabbit polyclonal TRIP13 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TRIP13 gene.
Western blot of TRIP13 Antibody in K562, HeLa, 293 cell line lysates (35 ug/lane). TRIP13 (arrow) was detected using the purified antibody.
TRIP13 Antibody flow cytometry of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.