Anti-TDP-43 / TARDBP antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Fluorescent confocal image of HeLa cell stained with TARDBP Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TARDBP primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.

TARDBP Antibody western blot of HeLa cell line and mouse brain lysates (35 ug/lane). The TARDBP antibody detected the TARDBP protein (arrow).