Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody. NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). SPI1 immunoreactivity is localized to Nucleus significantly.

SPI1 Antibody western blot of WiDr cell line lysates (35 ug/lane). The SPI1 antibody detected the SPI1 protein (arrow).