Western blot analysis of extracts from various cell lines using PSMB8/LMP7 (D1K7X) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (The T2 cell line contains a homozygous deletion of PSMB8/LMP7 (13)).
Western blot analysis of extracts from HeLa and SW620 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, 72 hr; +), using PSMB8/LMP7 (D1K7X) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human PSMB5 (hPSMB5-Myc/DDK; +) or Myc/DDK-tagged full-length human PSMB8 (hPSMB8-Myc/DDK; +), using PSMB8/LMP7 (D1K7X) Rabbit mAb (upper) and DYKDDDDK Tag Antibody #2368 (lower).