Rpb1 CTD (4H8) Mouse mAb
| Name | Rpb1 CTD (4H8) Mouse mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 2629 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 4H8 |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Hamster, Drosophila, Yeast |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide containing 10 heptapeptide repeats [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] in which Ser5 is phosphorylated. |
| Description | Mouse Monoclonal |
| Gene | POLR2A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using Rpb1 CTD (4H8) Mouse mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 HeLa cells and either 10 μl of Rpb1 CTD (4H8) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human RPL30 Exon 3 Primers #7014, SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP ® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.