Western blot analysis of IRS-1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human IRS-1 transfected: sc-116569 (B,E) and lambda protein phosphatase (sc-200312A) treated human IRS-1 transfected: sc-116569 (C,F) 293T whole cell lysates. Antibodies tested include p-IRS-1 (Ser 636): sc-33957 (A,B,C) and IRS-1 (A-19): sc-560 (D,E,F).
p-IRS-1 (Ser 636): sc-33957. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic and nuclear staining of Purkinje cells and nuclear staining of cells in granular layer and cells in molecular layer.