Detection of Human and Mouse Immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-No. Lot2) used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
Detection of Human RBM9 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human RBM9 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody used for IP at 6 ug/mg lysate. RBM9 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated RBM9, -2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat RBM9 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-RBM9 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.