
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D5I5F) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or PFKFB2 (D5I5F) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D5I5F) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.

Confocal immunofluorescent analysis of LNCaP (high expression, left) and HepG2 (low expression, right) using PFKFB2 (D5I5F) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).