
Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody (M01), clone 5G4.Lane 1: PITX1 transfected lysate(34.1 KDa).Lane 2: Non-transfected lysate.

PITX1 monoclonal antibody (M01), clone 5G4 Western Blot analysis of PITX1 expression in HeLa NE.

Immunoprecipitation of PITX1 transfected lysate using anti-PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.

Detection limit for recombinant GST tagged PITX1 is approximately 0.03 ng/ml as a capture antibody.

Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody (M01), clone 5G4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.